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phospho mk2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phospho mk2
    Phospho Mk2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+mk2/Phospho-MAPKAPK-2+(Thr334)+Rabbit+mAb/pm41916429-80-30-32
    Average 95 stars, based on 320 article reviews
    phospho mk2 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    other:

    Article Title: The p38/MK2 Pathway Functions as Chk1-Backup Downstream of ATM/ATR in G 2 -Checkpoint Activation in Cells Exposed to Ionizing Radiation.
    Article Snippet: The primary antibodies were anti-Chk1 (G-4) (Santa Cruz Biotechnology, Heidelberg, Germany), anti-pChk1-S345, anti-pChk1-S296, anti-HSP27, anti-pHSP27-S82, anti-MK2 and anti-pMK2T334 (all from Cell Signaling Technology, Leiden, The Netherlands), anti-Ku80 (GeneTex, Irvine, CA, USA), anti-Ku70 (N3H10) (GeneTex, Irvine, USA), and anti-GAPDH (MERCK, Darmstadt, Germany) at the corresponding dilutions (See Table S1 for details).

    Article Title: Degradation of MK2 with natural compound andrographolide: A new modality for anti-inflammatory therapy.
    Article Snippet: Anti-MK2 (Cat# 12155), anti-phospho-MK2 (Thr334, Cat# 3007), anti-phospho-MK2 (Thr222, Cat# 3316), anti-TTP (Cat# 71632), anti-KHSRP (Cat# 13398), anti-p38α (Cat# 9218), anti-p38 (Cat# 8690), anti-phospho-p38 (Thr180/Tyr182, Cat# 4511), anti-MKK6 (Cat# 8550), anti-HSP27 (Cat# 95357), anti-phospho-HSP27 (Ser82, Cat# 9709), anti-rabbit lgG (Cat# 2729) antibodies were purchased from Cell Signaling Technology (Beverly, MA).

    Article Title: The p38/MK2 Pathway Functions as Chk1-Backup Downstream of ATM/ATR in G 2 -Checkpoint Activation in Cells Exposed to Ionizing Radiation
    Article Snippet: The primary antibodies were anti-Chk1 (G-4) (Santa Cruz Biotechnology, Heidelberg, Germany), anti-pChk1-S345, anti-pChk1-S296, anti-HSP27, anti-pHSP27-S82, anti-MK2 and anti-pMK2-T334 (all from Cell Signaling Technology, Leiden, The Netherlands), anti-Ku80 (GeneTex, Irvine, CA, USA), anti-Ku70 (N3H10) (GeneTex, Irvine, USA), and anti-GAPDH (MERCK, Darmstadt, Germany) at the corresponding dilutions (See for details).

    Western Blot:

    Article Title: MUC1-C dependency in drug resistant HR+/HER2- breast cancer identifies a new target for antibody-drug conjugate treatment.
    Article Snippet: Total lysates and chromatin-bound protein prepared from nonconfluent cells were subjected to immunoblot analysis using antiMUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), antiMED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described25. .. Total lysates and chromatin-bound protein prepared from nonconfluent cells were subjected to immunoblot analysis using antiMUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), antiMED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described25. .. Nuclear proteins were incubated with anti-MUC1-C (#MA5–11202; Thermo Fisher Scientific), antiSRC-3 (2126S, CST), anti-MED1 (51613S, CST), Armenian Hamster IgG (ab18479, abcam) or Rabbit IgG (#NI01-100UG, Sigma), precipitated with Dynabeads Protein G (#10004D; Thermo Fisher Scientific) and analyzed as described57.

    Article Title: MUC1-C dependency in drug resistant HR+/HER2− breast cancer identifies a new target for antibody-drug conjugate treatment
    Article Snippet: .. Total lysates and chromatin-bound protein prepared from non-confluent cells were subjected to immunoblot analysis using anti-MUC1-C (#MA5–11202, 1:1000 dilution; Thermo Fisher Scientific, Waltham, MA, USA), anti-NaKATPase1 (3010S, 1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-LaminB1 (66095-1-Ig, 1:2000, Proteintech, Rosemont, IL, USA), anti-α-tubulin (2144S, 1:1000, CST), anti-H3 (9715S, 1:1000, CST), anti-ER (8644S, 1:1000, Cell Signaling Technology), anti-β-actin (A5441, 1:10,000 dilution, Sigma-Aldrich), anti-SRC-3 (2126S, 1:1000, CST), anti-pSRC3(S857) (PA5-106189, Thermo Fischer Scientific), anti-MK2 (12155S, 1:1000, CST), anti-MED1 (51613S, 1:1000, CST), anti-pMED1(T1457) (ab60950, 1:500, abcam), anti-CDK7 (2916S, 1:1000, CST), anti—pCDK7(T170) (ab155976, 1:1000, abcam), anti-pPol II(pRpb1)(S7) (13780S, 1:500, CST), anti-Pol II(Rpb1) (14958T, 1:1000, CST), anti-pRB(S780) (9307S, 1:1000, CST), anti-RB (9309S, 1:1000, CST) and anti-E2F1 (3742, 1:1000, CST) as described . ..

    Blocking Assay:

    Article Title: Newcastle disease virus suppresses antigen presentation via inhibiting IL-12 expression in dendritic cells
    Article Snippet: .. Following blocking, the PVDF membranes were reacted with anti-GAPDH, anti-p-Akt, anti-Akt, anti-p-p38, anti-p38, anti-p-MK2, anti-MK2, anti-p-p65, anti-p65, and anti-IL-12p70 primary antibodies overnight at 4 ℃ and horseradish peroxidase (HRP)‍-conjugated secondary antibodies (CST, MA, USA) for 45 min at room temperature. ..



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    Image Search Results


    Effect of SP600125, SB203580, or PD98059 on 3.5 GHz EMF-induced growth suppression of BV2 cells and phosphorylation of JNK-1/2, p38 MAPK (MK2), or ERK-1/2 in BV2 cells. ( A ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), a JNK-1/2 inhibitor, SB203580 (25 μM), a p38 MAPK inhibitor, or PD98059 (50 μM), an ERK-1/2 inhibitor for 2 h. The number of surviving cells was analyzed using cell count analysis. Data represent the mean ± SE of three independent experiments. * p < 0.01 compared with the values of the control (no 3.5 GHz EMF exposure). # p < 0.01 compared with the values of 3.5 GHz EMF exposure (no drug), as determined by one-way ANOVA followed by Sidak’s post hoc test. ( B ) A representative image of morphological changes in the conditioned cells in ( A ). ( C ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), SB203580 (25 μM), or PD98059 (50 μM) for 2 h. Whole-cell lysates from the conditioned cells were prepared and analyzed using Western blotting with antibodies.

    Journal: Current Issues in Molecular Biology

    Article Title: Mitigation of 3.5 GHz Electromagnetic Field-Induced BV2 Microglial Cytotoxicity by Polydeoxyribonucleotide

    doi: 10.3390/cimb47060386

    Figure Lengend Snippet: Effect of SP600125, SB203580, or PD98059 on 3.5 GHz EMF-induced growth suppression of BV2 cells and phosphorylation of JNK-1/2, p38 MAPK (MK2), or ERK-1/2 in BV2 cells. ( A ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), a JNK-1/2 inhibitor, SB203580 (25 μM), a p38 MAPK inhibitor, or PD98059 (50 μM), an ERK-1/2 inhibitor for 2 h. The number of surviving cells was analyzed using cell count analysis. Data represent the mean ± SE of three independent experiments. * p < 0.01 compared with the values of the control (no 3.5 GHz EMF exposure). # p < 0.01 compared with the values of 3.5 GHz EMF exposure (no drug), as determined by one-way ANOVA followed by Sidak’s post hoc test. ( B ) A representative image of morphological changes in the conditioned cells in ( A ). ( C ) BV2 cells were exposed to 3.5 GHz EMFs without or with SP600125 (25 μM), SB203580 (25 μM), or PD98059 (50 μM) for 2 h. Whole-cell lysates from the conditioned cells were prepared and analyzed using Western blotting with antibodies.

    Article Snippet: The primary antibodies, including eukaryotic initiation factor-2α (eIF-2α) (cat. no. 9722), phosphorylated (p)-extracellular signal-regulated protein kinase-1/2 (p-ERK-1/2) (T202/Y204) (cat. no. 9101), ERK-1/2 (cat. no. 9102), p-JNK-1/2 (T183/Y185) (cat. no. 9251), JNK-1/2 (cat. no. 9252), and p-p38 MAPK (T180/Y182) (cat. no. 9211) p38 MAPK (cat. no. 9212), p-MK2 (T334) (cat. no. 3007), and T-MK2 (cat. no. 3042), were obtained from Cell Signaling Technology, Inc. (Beverly, MA, USA).

    Techniques: Phospho-proteomics, Cell Counting, Control, Western Blot